Off-the-shelf sequences
AAV-like ssDNA
GT01 (3648 nt):
AAV2 ITRs, chicken beta-actin promoter, tdTomato, WPRE, SV40 poly(A) signal
GT02 (2041 nt):
AAV2 ITRs, CMV enhancer, CMV promoter, EGFP, SV40 poly(A) signal
GT03 (2985 nt):
AAV2 ITRs, hApoE hepatic control region, human alpha 1 antitrypsin promoter, hAAT 5' UTR, MVN intron, Human factor IX, bGH poly A
Sequences available upon request
Gene editing ssDNA
Available in linear and circular formats
GE01 (2204 nt): TRAC Locus
[5’Homology arm][GSG-P2A][eGFP][extended bGH polyA][3’homology arm]
KI template that expresses P2A-eGFP upon correct knock in. No promoter.
GT02 (2377 nt): TRAC Locus
[5’Homology arm][ef1as (taken from ef1as-Cas9 (Addgene: 110837][eGFP][extended bGH polyA][3’homology arm]
KI template that expresses EF1As-eGFP.
GT03 (2859 nt): AAVS1 Locus
[5’Homology arm][ef1as (taken from ef1as-Cas9 (Addgene: 110837][eGFP][extended bGH polyA][3’homology arm]
KI template that expresses EF1As-eGFP.
Sequences available upon request