Off-the-shelf sequences
| AAV-like ssDNA | Gene editing ssDNA, linear and circular | ||
|---|---|---|---|
| GT01 (3648 nt) | AAV2 ITRs, chicken beta-actin promoter, TdTomato, WPRE, SV40 poly(A) signal | GE01 (2204 nt) TRAC locus | 5' Homology arm, GSG-P2A, eGFP, extended bGH poly(A), 3' Homology arm |
| GT02 (2041 nt) | AAV2 ITRs, CMV enhancer, CMV promoter, EGFP, SV40 poly(A) signal | GE02 (2377 nt) TRAC locus | 5' Homology arm, ef1As-Cas9, eGFP, extended bGH poly(A), 3' Homology arm |
| GT03 (2985 nt) | AAV2 ITRs, hApoE hepatic control region, human alpha 1 antitrypsin promoter, hAAT 5' UTR, MVN intron, Human factor IX, bGH poly(A) | GE03 (2859 nt) AAVS1 locus | 5’ Homology arm, ef1as (taken from ef1as-Cas9), eGFP, extended bGH poly(A) 3’ Homology arm |
Sequences available upon request